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SKU: 2593-610M Category:

Product details

Synonyms = BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Antibody type = Recombinant Mouse monoclonal / IgG1

Clone = MSVA-610M

Positive control = Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.

Negative control = Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.

Cellular localization = Cytoplasmic

Reactivity = Human


Application = Immunohistochemistry
Dilution = 1:100 – 1:200
Intended Use = Research Use Only

Relevance of Antibody

Cytokeratin 10 is expressed in keratinizing squamous epithelial cells

Biology Behind

Cytokeratin 10 (CK10), also termed keratin 10 (KRT10) is a type I acidic high molecular weight keratin protein encoded by the KRT10 gene located 17q21. It dimerizes with the type I keratin 1 and forms intermediate filaments that primarily shape the cytoskeleton of specific epithelial cells. In these cells, KRT10 is part of the cytoskeletal scaffold which contributes to the cell architecture and provides the cells with the ability to withstand mechanical stress. Mutations in CK10 can cause epidermolytic hyperkeratosis or bullous congenital ichthyosiform erythroderma of Brocq.

Staining Pattern in Normal Tissues

Cytokeratin 10 staining pattern in Normal Tissues with antibody MSVA-610M (images are shown in our “Normal Tissue Gallery”)

Brain Cerebrum Negative.
  Cerebellum Negative.
Endocrine Tissues Thyroid Negative.
  Parathyroid Negative.
  Adrenal gland Negative.
  Pituitary gland Negative.
Respiratory system Respiratory epithelium Negative.
  Lung Negative.
Gastrointestinal Tract Salivary glands Negative.
  Esophagus Usually negative.
  Stomach Negative.
  Duodenum Negative.
  Small intestine Negative.
  Appendix Negative.
  Colon Negative.
  Rectum Negative.
  Liver Negative.
  Gallbladder Negative.
  Pancreas Negative.
Genitourinary Kidney Negative.
  Urothelium Negative.
Male genital Prostate Negative.
  Seminal vesicles Negative.
  Testis Negative.
  Epididymis Negative.
Female genital Breast Negative.
  Uterus, myometrium Negative.
  Uterus, ectocervix Usually negative.
  Uterus endocervix Negative.
  Uterus, endometrium Negative.
  Fallopian Tube Negative.
  Ovary Negative.
  Placenta early Negative.
  Placenta mature Negative.
  Amnion Negative.
  Chorion Negative.
Skin Epidermis Strong CK10 staining of all suprabasal cell layers of the keratinizing squamous epithelium. The basal cell layer of the epidermis is CK10 negative.
  Sebaceous glands Moderate to strong CK10 staining sebaceous glands and suprabasal cells of hair follicles.
Muscle/connective tissue Heart muscle Negative.
  Skeletal muscle Negative.
  Smooth muscle Negative.
  Vessel walls Negative.
  Fat Negative.
  Stroma Negative.
  Endothelium Negative.
Bone marrow/lymphoid tissue Bone marrow Negative.
  Lymph node Negative.
  Spleen Negative.
  Thymus Moderate to strong CK10 staining of keratinizing squamous epithelial cells the central areas of corpuscles of Hassall’s.
  Tonsil Moderate to strong CK10 staining of a fraction of superficial keratinizing squamous epithelial cells in the of tonsil crypts.


CK10 is always strongly expressed in all suprabasal cell layers of the keratinizing squamous epithelium of the skin as well as in associated sebaceous glands and hair follicles. The basal cell layer of the epidermis is strictly CK10 negative. Strong CK10 expression is also regularly observed in a superficial keratinizing fraction of cells in the squamous epithelium of tonsil crypts and the central areas of corpuscles of Hassall’s. CK10 is usually absent in non-keratinizing squamous epithelium such as of the ectocervix, tonsil surface, esophagus, oral cavity and the lip. However, a variable fraction of suprabasal cells or groups of cells in these non-keratinizing squamous epithelia may occasionally show a weak, moderate, or even strong KRT10 staining. KRT10 immunostaining is absent in urothelium and transitional epithelium of the anal mucosa. CK10 immunostaining was absent in gastrointestinal epithelium, gallbladder, liver, pancreas, lung, fallopian tube, endocervical glands of the uterus, ovary, placenta, lymphatic, hematopoetic and mesenchymal tissues, the pituitary gland, and the brain.

These findings are largely comparable to the RNA and protein data summarized in the Human Protein Atlas (Tissue expression Cytokeratin 10) 



Suggested positive tissue control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.

Suggested negative tissue control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.


Normal tissue gallery

Staining Pattern in Relevant Tumor Types

KRT10 immunostaining almost exclusively occurs in squamous cell carcinomas of various sites of origin. The rate of KRT10 positive cases may vary depending on the histologic tumor grade. Some of the KRT10 positive squamous cell carcinomas show – similar to the situation in normal skin – absence of expression in the basal cell layers.

The TCGA findings on Cytokeratin 10 RNA expression in different tumor categories have been summarized in the Human Protein Atlas.


Cancer tissue gallery

Compatibility of Antibodies

No data available at the moment

Protocol Recommendations

IHC users have different preferences on how the stains should look like. Some prefer high staining intensity of the target stain and even accept some background. Others favor absolute specificity and lighter target stains. Factors that invariably lead to more intense staining include higher concentration of the antibody and visualization tools, longer incubation time, higher temperature during incubation, higher temperature and longer duration of the heat induced epitope retrieval (slide pretreatment). The impact of the pH during slide pretreatment has variable effects and depends on the antibody and the target protein. Accordingly, multiple different protocols can generate identical staining results.


All images and data shown here and in our image galleries were obtained by the manual protocol described below. Other protocols resulting in equivalent staining are described as well.


Manual protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH7,8 Target Retrieval Solution buffer. Apply MSVA-610M at a dilution of 1:150 at 37°C for 60 minutes.  Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.


Impact of pH


MSVA-610M results in strongest staining if pH9,0 is used for slide pretreatment. pH7,8 is acceptable but lower pH results in a significant reduction of sensitivity. 

Potential Research Applications

  • The diagnostic utility of reduced CK10 expression in the skin is not known.
  • The diagnostic utility of CK10 neo-expression in non-keratinizing squamous epithelium of ectocervix, esophagus, oral cavity and lip is not known.
  • The prognostic role of CK10 expression in squamous cell carcinoma is unknown.

Evidence for Antibody Specificity in IHC

Utility of MSVA-610M is documented by a staining pattern that exactly matches the data summarized in the protein atlas. A strong positive KRT10 staining almost exclusively seen in the skin. All other tissues showing weak or occasional KRT10 staining are also squamous epithelia. In addition, all tissues known to not express KRT10 including those notorious for non-specific IHC background such as kidney and colonic mucosa are completely KRT10 negative.



Normal tissue gallery