Normal Tissue Gallery Cystatin A

Adrenal gland

Aorta, media

Appendix, mucosa

Appendix, muscular wall

Bone marrow – Weak to moderate Cystatin A staining of a subset of cells.

Breast

Bronchus, mucosa – Cystatin A staining is seen in mucinous cells of bronchial glands and in few cells of the respiratory epithelium

Cerebellum (granule cell layer, white matter)

Cerebellum (molecular layer, Purkinje cell layer, granule cell layer, white matter)

Cerebrum, grey matter

Cerebrum, white matter

Colon descendens, mucosa

Colon descendens, muscular wall

Duodenum, Brunner gland

Duodenum, mucosa

Epididymis – Weak to moderate intensity Cystatin A staining of basal.

Esophagus, squamous epithelium – Strong Cystatin A immunostaining in suprabasal epithelial cell layers.

Fallopian tube, mucosa

Fat

Gallbladder, epithelium

Heart muscle

Ileum, mucosa – Strong Cystatin A immunostaining in dendritic cells of a germinal centre.

Kidney, cortex

Kidney, medulla

Liver – Cystatin A immunostaining is not seen in this liver sample.

Liver – Faint Cystatin A immunostaining of hepatocytes showing a zonal distribution.

Lung

Lymph node – Strong Cystatin A immunostaining in dendritic cells of germinal centres.

Ovary, stroma

Pancreas

Parathyroid

Parotid gland

Pituitary gland, anterior lobe

Pituitary gland, posterior lobe

Placenta (amnion and chorion)

Pregnant uterus (decidua)

Placenta, early

Placenta, mature

Prostate – Moderate intensity Cystatin A staining of basal cells.

Rectum, mucosa

Seminal vesicle – Weak to moderate intensity Cystatin A staining of basal cells.

Sinus paranasales

Skeletal muscle

Skin – Cystatin A immunostaining is nuclear and cytoplasmic. It predominates in the granular cell layer and decreases towards the basal cell layer

Spleen – Weak Cystatin A immunostaining in granulocytes.

Stomach, antrum – Faint basal cytoplasmic Cystatin A immunostaining of surface epithelial cells.

Stomach, corpus – Faint basal cytoplasmic Cystatin A immunostaining of surface epithelial cells

Sublingual gland – Weak to moderate Cystatin A staining in myoepithelial cells and some mucinous glands.

Testis

Thymus – Strong Cystatin A immunostaining of a fraction of cells in corpuscles of Hassall’s.

Thyroid gland

Tonsil – Strong Cystatin A immunostaining of crypt epithelium and of dendritic cells. Adjacent lymphocytes also show weak staining. This may represent a tissue contamination due to the very high levels of target protein in this tissue sample.

Tonsil, surface epithelium – Intense Cystatin A immunostaining occurs across all epithelial cell layers. A weaker staining is also seen in subepithelial inflammatory cells and endothelia of subepithelial vessels. Endothelial cell staining is only seen in vessels adjacent to strongly positive epithelial tissues.

Tonsil, surface epithelium – Intense Cystatin A immunostaining occurs across all epithelial cell layers. A weaker staining is also seen in subepithelial inflammatory cells. This may represent tissue contamination in case of very high levels of target protein or cystatin A secretion.

Urinary bladder, muscular wall

Urinary bladder, urothelium

Uterus, ectocervix – Strong Cystatin A immunostaining in suprabasal epithelial cells.

Uterus, endocervix

Uterus, endometrium (proliferation)

Uterus, endometrium (secretion)

Uterus, myometrium